The Youth Team were beaten 3-2 by Carlise United in the EFL Youth Alliance League on Saturday.
Darren Ehimamiegho and Oscar Kelly scored Dale's goals as the Youth Team were narrowly beaten away from home.
That result leaves Dale in sixth place in the league, still with a number of games on the teams above them.
Lee Thorpe's side will be looking to bounce back as they travel to Oldham Athletic this weekend.